本文件提供了与生物技术细胞计数相关的标准术语。本文件描述了悬浮液中细胞的计数（通常是细胞浓度）和粘附到基质上的细胞（通常是细胞的面积密度）。本文件提供了一般计数方法（包括总计数和差分计数、直接计数和间接计数）以及适合目的的方法选择、测量过程中的可变性来源以及数据分析和报告的主要注意事项。 本文件适用于所有细胞类型的计数——哺乳动物和非哺乳动物（如细菌、酵母）细胞。 请注意，目前存在几种特定于部门或应用的细胞计数的国际和国家标准。当适用时，用户可以在其范围内操作时参考现有标准（例如，特定测量技术或应用）。

This document provides standard terminology related to cell counting for biotechnology. This document describes counting of cells in suspension (generally cell concentration) and cells adhered to a substrate (generally area density of cells). This document provides key considerations for general counting methods (including total and differential counting, and direct and indirect counting) as well as for fit for purpose method selection, sources of variability in the measurement process, and data analysis and reporting.

This document is applicable to the counting of all cell types – mammalian and non-mammalian (e.g. bacteria, yeast) cells.

NOTE            Several sector or application-specific international and national standards for cell counting currently exist. When applicable, the user can consult existing standards when operating within their scope (e.g. specific measurement techniques or applications).